Fig. 1: Pro-inflammatory colonic macrophage subpopulations increase with high-fat diet.
From: Targeting colonic macrophages improves glycemic control in high-fat diet-induced obesity
Wild-type mice were fed either a coconut-based HFD (red circles) or a control diet (black square) for up to 12 weeks: a Representative flow cytometry plots and distribution of colonic macrophage (c-Mac) subpopulations P1–P5 in a mouse fed chow. b Intraperitoneal glucose tolerance test (IPGTT), body weight, and insulin after 1 week (wk) HFD (n = 10) or chow (n = 8). c Gene expression in colon tissue of mice fed a HFD for 1 wk relative to controls. d Fold change in absolute numbers (♯) of monocyte-derived CCR2+ (pro-inflammatory P1, P2, intermediate P3) and anti-inflammatory/resident CCR2- (P4-P5) c-Macs. e Gene expression in adipose tissue of HFD-fed mice relative to controls. f Fold change of adipose tissue macrophages (#ATMs/g) and their subpopulations (double negative DN, M1a, M1b, M2) upon HFD. g Fold change of plasma TNF and IL-6 upon HFD. Statistical data are expressed as mean ± SEM. Data are representative of one experiment (b) or 2–6 (c–g) independent experiments, with each data point representing an individual mouse. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Mann–Whitney U test with two-tailed distribution.
