Fig. 5: Colonic macrophages upon HFD show an interferon signature and altered mitochondrial metabolism with activated mTOR as a common regulator.
From: Targeting colonic macrophages improves glycemic control in high-fat diet-induced obesity
Wild-type mice were fed a coconut-based HFD for 1 week (wk) and compared to mice fed chow: a, b Principal component analysis (PCA) (a) and relative proportion of colonic macrophages (c-Macs) (b). c Average gene expression of specific marker genes in c-Mac clusters. d Up- (red) or down-regulated (blue) Molecular Signatures Database (MSigDB) hallmark pathways after 1 wk of HFD for cluster P1 (FDR < = 0.1). e Geometric mean fluorescent intensity (gMFI) of mitochondrial mass (MitoGreen), potential (MitoRed), and reactive oxygen status (MitoSOX) of total c-Macs and their subpopulations from mice fed 1 wk a HFD (red circles) compared chow-fed controls (black squares). f mTOR activation was measured by gMFI of phosphorylated S6 (pS6) and Akt (pS475) in c-Macs. Statistical data are expressed as mean ± SEM. Data are representative of two replicates (n = 2 per group) (a–d) and one (e) or two (f) independent experiments, with each data point representing one cell (a) or one individual mouse (e–g). *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Mann–Whitney U test with two-tailed distribution.
