Fig. 1: Characterization of sorghum transformation. | Communications Biology

Fig. 1: Characterization of sorghum transformation.

From: Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

Fig. 1

a–c Gene delivery capacity of ternary transformation system. Gene delivery was represented by the number of transgenic cells exhibiting YFP fluorescence on the surface of sorghum embryos. d–f Tissue culture response represented by callus proliferation after two weeks of Agrobacterium infection on the multi-purpose medium without selection. g–i YFP images of early-stage somatic embryo formation (indicated by the arrows) induced by morphogenic gene expression on the surface of immature scutella. j–l Shoot regeneration after four-week of maturation. m–o The pleiotropic impacts of morphogenic gene expression on single-copy QEs. a, d, g, j and m Tx430. b, e, h, k, and n Tx623. c, f, i, l and o Tx2752.

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