Fig. 3: STK25 regulates oxidative stress in human aortic endothelial and smooth muscle cells. | Communications Biology

Fig. 3: STK25 regulates oxidative stress in human aortic endothelial and smooth muscle cells.

From: Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective

Fig. 3

hASMCs and hAECs were transfected with STK25 siRNA or NTC siRNA. a–c Representative images of cells stained with DHE (pink) or processed for immunofluorescence with anti-8-oxoG (red) or anti-E06 (red) antibodies; nuclei stained with DAPI (blue). Quantification of the staining. d, f Cellular ROS content. e, g Cellular NO content. In (a–c), the scale bars represent 100 µm. Data in (a–c) are mean ± SEM from 6-8 wells per group; data in (d–g) are mean ± SEM from 20 wells per group. OA, oleic acid. *P < 0.05, **P < 0.01, ***P < 0.001 for STK25 siRNA vs. NTC siRNA.

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