Fig. 5: STK25 modulates aortic oxidative and ER stress in vivo.

Atherosclerotic Stk25 knockout and transgenic mice, as well as wild-type littermates, were generated by gene transfer of gain-of-function mutant of PCSK9 combined with an atherogenic western-type diet. Saline-injected wild-type mice fed the western-type diet were included as controls. a–d Representative images of aortic sinus sections processed for immunofluorescence with anti-8-oxoG (green), anti-E06 (green), anti-KDEL (green), or anti-CHOP (red) antibodies; nuclei stained with DAPI (blue). Dashed line and L indicate lumen. The scale bars represent 50 µm. Negative controls by excluding primary antibody or by substituting a primary antibody by an equivalent concentration of the corresponding Ig isotype (Supplementary Fig. 11a, b) did not detect any positive signal, confirming the specificity of the immunostaining. Quantification of the staining. Data are mean ± SEM from 6-7 mice per group. L, lumen. **P < 0.01, ***P < 0.001 for knockout vs. wild-type mice and transgenic vs. wild-type mice.