Fig. 8: Dominant-negative effect of G2^R398W and G2^T354M on transactivation activity.

a–c Scatter dot plots of the transcriptional activity measured by luciferase reporter assay in HEK293T cells following transfection with luciferase reporter constructs containing single (a) and tandem (b, c) GATA motifs together with the respective GATA2 expression constructs at doses of 100, 200, and 300 ng/well. AGATAAGATAA-type (middle) and AGATAAAGATAA-type (bottom) tandem GATA constructs were used for transient reporter/effector cotransfection assays. Upper and middle panels, n = 5 biologically independent samples and bottom panel, n = 4. d–f Similar sets of experiments in which 100 ng of the G2^WT expression construct and luciferase reporter construct containing single (d) and tandem (e, f) GATA motifs were concomitantly transfected with G2^WT, G2^R398W, or G2^T354M expression constructs at doses of 0, 100, and 200 ng/well. AGATAAGATAA-type (middle) and AGATAAAGATAA-type (bottom) tandem GATA constructs were used for transient reporter/effector cotransfection assays. Upper and middle panels, n = 5 biologically independent samples and bottom panel, n = 8. The average luciferase activity of G2^WT at a dose of 100 ng/well was set to 1.0 in a–c, and fold changes of the average value of 100 ng of G2^WT are shown in d–f. *p < 0.05.