Fig. 2: Histological analysis of vascular development in endothelial-specific Med23-deletion mice. | Communications Biology

Fig. 2: Histological analysis of vascular development in endothelial-specific Med23-deletion mice.

From: Med23 supports angiogenesis and maintains vascular integrity through negative regulation of angiopoietin2 expression

Fig. 2

a Whole-mount immunofluorescence staining for PECAM and Med23 in E9.5 wild-type embryos. The boxed area indicates abundant expression of Med23 in the vasculature of the head. The arrowheads and asterisks indicate the areas where the Med23 signal is weak and where Med23 does not colocalize well with PECAM. Magnified views of the boxed regions are shown under the corresponding pictures. Scale bars for the upper panel, 2.5 mm. Scale bars for the bottom panel, 0.5 mm. b H&E staining to analyze the lumen size of the blood vessels in the head in E9.5 embryos. The asterisks indicate blood vessels. Scale bar, 200 μm. c Quantitation of blood vessel lumen size in the control and Med23-mutant mice in b. Nine mouse samples from each group were used for the statistical analysis. The values are the means ± SDs. ***p < 0.001. d PECAM staining to display the vascular network in the heads of control and Med23-mutant mice (E9.5). The asterisks indicate the intracranial artery, the open arrowheads indicate the venous plexus, and the solid arrowheads mark the intracranial arterial network. A poorly developed intracranial vasculature was easily identified in Med23-mutant mice. Scale bar, 200 μm. e Quantification of filopodia in in vivo retinal angiogenesis assays. n = 3 for each group. The values are the means ± SDs. ***p < 0.001. f PECAM staining of the in vivo-generated retinal vascular network. Magnified views of the boxed regions are shown under the corresponding pictures. Scale bars for the upper panel, 250 μm. Scale bars for the bottom panel, 100 μm. g Immunofluorescence images of the intersomitic vasculature. Both control and Med23f/f:Tie2-Cre embryos displayed normal vascular networks. Scale bar, 200 μm. h Histological images of the dorsal aorta in the transaxial plane. Scale bar, 100 μm. i Whole-mount immunofluorescence staining of the endocardium in E12.5 embryos (left; Scale bar, 300 μm) and histological analysis of E12.5 heart sections (right; Scale bar, 50 μm). The endothelial marker PECAM was labeled in cardiac tissues by immunofluorescence staining.

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