Fig. 4: Upregulation of Ang2 by Med23 knockdown.
a Heatmap of RNA-seq data from HUVECs with knockdown of Med23 or control HUVECs based on the log2 intensity (n = 3 for each group). b GO analysis of DEGs in HUVECs with Med23 knockdown or control HUVECs, ranked by the p value. c qRT–PCR assays to validate the upregulation of Ang2 caused by Med23 knockdown in HUVECs based on the RNA-seq data. n = 3 for each group. The values are the means ± SDs. **p < 0.01. d Enzyme-linked immunosorbent assays (ELISAs) to validate the enhanced secretion of Ang2 upon Med23 knockdown in HUVECs. n = 3 for each group. The values are the means ± SDs. **p < 0.01. e qRT–PCR assays to determine the upregulation of Ang2 in the head region caused by Med23 endothelial-specific deletion. Three embryos of the control and Med23f/f; Tie2-Cre from different litters at E12.5 were analyzed respectively. The qPCR data were normalized to the internal control β-actin, and the cDNAs were reverse-transcribed from the whole head RNAs. The values are the means ± SDs. *p < 0.05. f IF assays to determine the upregulation of Ang2 in the head region caused by Med23 endothelial-specific deletion at E12.5. The arrowheads indicate the positions of blood vessels. The dotted lines indicate the outlines of blood vessels. The white lines indicate the edge of samples. Scale bar, 200 μm. g Ang2 IF signal quantitation in (f). Three embryos of the control and Med23f/f; Tie2-Cre from different litters were analyzed respectively. The values are the means ± SDs. **p < 0.01.
