Table 2 Binding kinetics of the native and synthetic CODH-Ls as determined by SPR measurements.

From: Control of carbon monoxide dehydrogenase orientation by site-specific immobilization enables direct electrical contact between enzyme cofactor and solid surface

Analyte

Association rate, ka (M s−1)

Dissociation rate, kd (10−5s−1)

Equilibrium dissociation, KD (nM)

CODH-L

1245

6.88

55.22

CODH-LgbpN

4983

4.08

8.19

CODH-LgbpC

4511

12.11

26.77

CODH-LgbpNC

18258

3.45

1.89

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