Fig. 7: Sirt1 knockout reduced age-dependent changes of molecular pathways in aging HSCs. | Communications Biology

Fig. 7: Sirt1 knockout reduced age-dependent changes of molecular pathways in aging HSCs.

From: Loss of SIRT1 inhibits hematopoietic stem cell aging and age-dependent mixed phenotype acute leukemia

Fig. 7

a Hierarchical clustering of microarray gene expression in SP cells. b–d GSEA plots for ribosomal genes (b), peptide chain elongation (c), and electron transport chain/OXPHOS (d). The method for ranking genes was the default “Signal2Noise,” and the scoring scheme was also the default “weighted”. GSEA plots were ranked by NES and the full sets of results were provided in Supplementary Data 1 and 2. e OWT/YWT and OKO/OWT gene expression ratio of ribosomal genes analyzed by real time RT-PCR. f Real time RT-PCR analysis of top ranked OXPHOS genes in OWT and OKO HSCs. g Analysis of 18S + 28S rRNA of HSCs by Agilent Bioanalyzer. *P < 0.05; **P < 0.01. Error bars represented one standard deviation.

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