Fig. 8: SIRT1 knockout impacted multiple downstream effectors in aging HSCs. | Communications Biology

Fig. 8: SIRT1 knockout impacted multiple downstream effectors in aging HSCs.

From: Loss of SIRT1 inhibits hematopoietic stem cell aging and age-dependent mixed phenotype acute leukemia

Fig. 8

a Venn diagram analysis identified 250 genes that were up in OWT compared to YWT, but were down-regulated in OKO HSC cells. b Realtime RT-PCR validation of key genes identified in a. Geometric averaging of multiple control genes (β-actin, 18S RNA and CD45) was used to normalize data. c Trim26 mRNA in purified HSCs in independent aging cohorts (n = 7 each). d Trim26 and Sirt1 mRNA in spleen and heart using unfractionated cells. e Western blot analysis of MEF cell lysates. f, g Overlap of PHF20 target genes with genes down in OKO over OWT HSCs (f) and 222 annotated genes from 250 genes that were up in OWT vs YWT but down in OKO (g). *P < 0.05; **P < 0.01; ***P < 0.001. n.s. not significant. Error bars represented one standard deviation.

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