Fig. 5: Co-analysis of RA- and RA+ cells. | Communications Biology

Fig. 5: Co-analysis of RA- and RA+ cells.

From: Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency

Fig. 5

a (i) Representative plots of membrane potential in RA- and RA+ organoid derived CMs at Day 30 and (ii) quantifications of action potential duration (APD) and beating rates in the cells. For APD, n = 299 cells in RA-, N = 129 cells in RA+. For BPM, n = 298 cells in RA-, N = 124 cells in RA+. ****p < 0.0001, Student’s t test. b UMAP projection of single cells grouped by cell type. c UMAP projections of single cells colored by the expression pattern of representative cardiac lineage genes. d UMAP projection of CMs grouped by (i) treatments, (ii) differentiation methods, and (iii) stages. e UMAP projections of CMs colored by the expression pattern of atrial and ventricular marker genes. f Expression levels of atrial and ventricular marker genes in each conditioned CMs. N = 592 D15_RA-, 563 D15_RA+, 927 D30_RA-, 1273 D30_RA+. ns p > 0.05, *p < = 0.05, ****p < = 0.0001, Bonferroni corrected p values from Wilcox test, exact p-values and sample numbers available in a Source data file.

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