Fig. 9: Study of EA defects in a mutant isogenic line using random forest predictions. | Communications Biology

Fig. 9: Study of EA defects in a mutant isogenic line using random forest predictions.

From: Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency

Fig. 9

a UMAP projections of mutant RA- and RA+ single cells at day 30 grouped by (i) cell types, (ii) cell line, and (iii) differentiation method. b UMAP projections of day 30 mutant RA- and RA+ single cells with Anomalies removed grouped by (i) cell types, (ii) treatment, and (iii) predicted cell types and zone. c Sankey diagram of prediction results of mutant RA- and RA+ cells at day 30 (Anomalies removed). Table provides cell type and conditional zone accuracies. d Expression levels of genes used to make prediction decisions that either correlate with the predictions. N = 1087 A_RA-, 407 V_RA-, 1931 A_RA+, 173 V_RA+. ****p ≤ 0.0001, Bonferroni corrected p values from Wilcox test, exact p-values and sample numbers available in a Source data file. e The results of side predictions in mutant cells at day 30. f Gene pathways that differentially enrich in the wild-type and mutant (predicted) CMs.

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