Fig. 6: Conformational analyses of the S4/S5/S6 transmembrane portion of mHCN2.
a The gate region of the mHCN2 channel pore with only two opposite subunits is shown. b Distance measurements between the Cβ-atoms of two opposite amino acids that form the gate. c Residue-wise root mean square fluctuations (RMSF) of gate-forming amino acids. All non-hydrogen side-chain atoms were considered. d The close-up view depicts the suggested interaction between D443 on subunit i (blue) and R339 on i + 1 (green). e Average occurrence frequency of salt bridge interactions between residues depicted in panel (d). In a and d, relevant amino acids are shown as sticks. In b, c, and e, the individual data points are shown as circles and the mean values as black lines (n = 40 in b and n = 80 in c and e). The boxes denote the range from the 25th to 75th percentile and include 50% of the data points. The whiskers denote the 5th to 95th percentile and include 90% of the data points (*p < 0.05; **p < 0.01; n.s. not significantly different; p value by t-test).
