Fig. 4: Th2-predominant response promotes tissue/peripheral eosinophilia and humoral response. | Communications Biology

Fig. 4: Th2-predominant response promotes tissue/peripheral eosinophilia and humoral response.

From: Functional IKK/NF-κB signaling in pancreatic stellate cells is essential to prevent autoimmune pancreatitis

Fig. 4

a CD3, CD4 and CD8 stainings on pancreatic tissues. Scale bar: 50 µm. b Expression of Th1, Th2 and Th17 cytokines analyzed by qPCR (n = 8). c FACS analysis showing an increase in the CD45+CD3+CD4+IL17A+ population in the NEMOΔCol1a2 pancreata. d B220 and CD138 stainings showing increased B cells/plasma cells in the NEMOΔCol1a2 pancreata. Scale bar: 50 µm. e Quantification of B220+ cells per field (n = 3). f Measurement of the circulating IgM (3 weeks: n = 8; 6 weeks: n = 6) and IgG (n = 7 for all time points) levels by ELISA on serum and the absorbance was determined. g Serum ANA levels measured by ELISA (n = 8). Whiskers: Min to Max. T-test (two-tailed): *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All n numbers represent biological replicates.

Back to article page