Fig. 5: Ablation of NEMO in PSCs leads to stronger fibrosis after caerulein-induced pancreatitis. | Communications Biology

Fig. 5: Ablation of NEMO in PSCs leads to stronger fibrosis after caerulein-induced pancreatitis.

From: Functional IKK/NF-κB signaling in pancreatic stellate cells is essential to prevent autoimmune pancreatitis

Fig. 5

a AZAN trichrome staining showing an increase in extracellular matrix deposits (blue). Scale bar: 100 µm. b Quantification of AZAN staining as a percentage of area (n = 8). c Co-staining of RFP/CD45 and RFP/αSMA on the pancreatic tissue from the NEMOΔCol1a2.RFP reporter mice. Scale bar: 25 µm. d Expression of pro-fibrogenic genes analyzed by qPCR (Saline: n = 4; 3 weeks: n = 8). e Expression of Il6 in pancreata analyzed by qPCR (n = 8). f Immunoblot of p-STAT3 and total STAT3 levels from pancreatic protein extracts. g Ratio of p-STAT3 to GAPDH band intensities (n = 7). h Immunoblot of components of the MAPK pathway on pancreatic protein extracts. WT: NEMOWT; NEMO: NEMOΔCol1a2. Whiskers: Min to Max. (b, e, g) T-test (two-tailed). d One-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All n numbers represent biological replicates.

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