Fig. 8: PSCΔNEMO cells promote eosinophilia through the secretion of CCL24. | Communications Biology

Fig. 8: PSCΔNEMO cells promote eosinophilia through the secretion of CCL24.

From: Functional IKK/NF-κB signaling in pancreatic stellate cells is essential to prevent autoimmune pancreatitis

Fig. 8

a Stainings of Vim, CD45 and RFP on freshly purified PSCs isolated from NEMOΔCol1a2.RFP mice (3-week time point). b, c PSCs formed fibroblastoid morphology and express RFP. RFP and nestin stainings on PSCs after 24 h in culture. d Immunoblot of NEMO on PSC protein extracts. TOM20: loading control. WT: PSCWT; NEMO: PSCΔNEMO. e, f Gene expressions in PSCs analyzed by qPCR (PSCWT: n = 3; PSCΔNEMO: n = 4). g Measurement of CCL24 level by ELISA from culture media collected at 48 h (n = 8). Whiskers: Min to Max. T-test (two-tailed): *p < 0.05; **p < 0.01. h, i Transwell migration assay indicating the fold change of the immune cells towards different media. Media: PSCWT (WT) and PSCΔNEMO (NEMO) conditioned media (72 h); media with an addition of CCL24 ligand or neutralization with CCL24 antibody (ab). n = 3. One-way ANOVA: *p < 0.05. j Staining of Siglec-F on the transmigrated cells. DAPI: nuclei. Scale bar: 100 µm. All n numbers represent biological replicates.

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