Fig. 5: Histological and ultrastructural characterization of tau SPAM mice pathological tau inclusions.

IHC staining of tau pathological inclusions with (a) anti-ubiquitin and (b) anti-p62/sequestosome-1. In 6-month-old tau SPAM mice, tau inclusions were also (c) argyophillic as stained with Campbell–Switzer silver stain and (d) readily labeled with Thioflavin S. Scale bars are 50 μm for main image and 25 μm for inset. e Immuno-EM analysis revealed that tau aggregates are composed of ultrastructural tau filaments as detected and labeled with 3026 antibody against total tau. Scale bar = 500 nm. f Magnified image shows pathogenic tau within straight filamentous structures. Scale bar = 100 nm for main image and inset.