Fig. 7: Astrogliosis is delayed compared with tau-inclusion formation in tau SPAM mice. | Communications Biology

Fig. 7: Astrogliosis is delayed compared with tau-inclusion formation in tau SPAM mice.

From: Pathogenic tau recruits wild-type tau into brain inclusions and induces gut degeneration in transgenic SPAM mice

Fig. 7

a IHC with anti-GFAP antibody for reactive astrocytes was used to stain brain samples from tau SPAM mice and nTg mice at ages 2, 4, and 6 months. GFAP immunoreactivity was significantly increased in the (b) hippocampus [one-way ANOVA, F (5, 42) = 3.591, p = 0.0086], (c) amygdala [one-way ANOVA, F (5, 42) = 4.317, p = 0.0029], and (d) brainstem [one-way ANOVA, F (5, 42) = 10.84, p < 0.0001] at 6 months. (e) Cortex and (f) thalamus did not show significant increase in anti-GFAP immunoreactivity (N = 8, 4 M, 4 F for each time point). Scale bar = 100 μm. GFAP staining was quantified with Aperio algorithms and compared using one-way ANOVA with Dunnett’s test. Error bars represent standard errors of the mean. ***p < 0.001, **p < 0.01, *p < 0.05, ns not statistically significant.

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