Fig. 1: α-Cre locus identification. | Communications Biology

Fig. 1: α-Cre locus identification.

From: Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons

Fig. 1

a Schematic of the vector used to make α-Cre mice (including Pax6 α-enhancer, Pax6 P0 promoter, Cre, IRES, GFP, Hbb intron, and SV40 poly A sequence), showing positions of primers within Cre used for TLA. b TLA sequence coverage across the mouse genome using primer set 1. The chromosomes are indicated on the y-axis, the chromosomal position on the x-axis. Similar results were obtained with primer set 2. Peak at Chromosome 7 shows inserted sequence (black circle). c Regional coverage of the α-Cre insertion site on Chromosome 7 using both sets of primers, showing a 27 kb deletion and spanning 2 Mb. Some RefSeq genes flanking the insertion site (version Mm10) are also indicated.

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