Fig. 4: α-Cre activity in the retina. | Communications Biology

Fig. 4: α-Cre activity in the retina.

From: Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons

Fig. 4

a Horizontal sections of α-Cre;Ai14 head at E12, showing tdTomato in the nasal and temporal peripheral retina as well as RGC axons in the optic nerve and optic chiasm. b X-gal staining of α-Cre;R26R eyeball at E14, showing α-Cre activity in the nasal and temporal peripheral retina. c, d X-gal staining of α-Cre;R26R whole retina at P0 (c), and tdTomato fluorescence in α-Cre;Ai14 at P18 both showing α-Cre activity in the nasal and temporal peripheral retina but not the dorsal and ventral retina. e Horizontal sections of α-Cre;Ai14 retina at E12, showing α-Cre expression (anti-Cre immunofluorescence), GFP expression (anti-GFP immunofluorescence), and lineage tracing (tdTomato fluorescence) in the nasal and temporal peripheral retina. f Horizontal sections of α-Cre;Ai14 retina at E16, showing Cre and GFP in amacrine cells (white arrows). g AP2a staining (red) of P8 α-Cre retina, white arrows show typical GFP+; AP2a+ cells. h Ki67 staining (red) of E16 α-Cre retina; white arrows show typical GFP+;Ki67- cells. The white boxes in e, f represent the expression areas of Cre, GFP or tdTomato in retinal progenitors, respectively. ON, optic nerve; ONL, outer nucleus layer; INL, inner nucleus layer; GCL, ganglion cell layer; NBL, neuroblastic cell layer. Scale bars: a 200 µm; c–h 50 µm.

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