Fig. 5: SHORT allows multiple rounds of staining, imaging, and stripping of large portions of human brain slices. | Communications Biology

Fig. 5: SHORT allows multiple rounds of staining, imaging, and stripping of large portions of human brain slices.

From: 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

Fig. 5

a Seven different antibodies were used across 3 sequential rounds of immunostaining, with SST employed as fiducial markers in each round. Round 1: PV—SST—VIP; round 2: CR- SST—NeuN; round 3: VIM—SST—GFAP. Scale bar = 1 mm. b The merged image shows a particular of the white and grey matters labeled with VIP, SST, PV, CR, NeuN, GFP, VIM. MIP of 70 slices. Scale bar = 100 μm.

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