Fig. 2: Transcriptional regulation of SUN1, SUN2 and LAP2 by PREP1. | Communications Biology

Fig. 2: Transcriptional regulation of SUN1, SUN2 and LAP2 by PREP1.

From: The transcription factor PREP1(PKNOX1) regulates nuclear stiffness, the expression of LINC complex proteins and mechanotransduction

Fig. 2

a, b Bar graph shows transcript levels of SUN1, SUN2, LMNA, LBR, LAP2 and SYNE2 in control (siLUC) and PREP1 depleted (siPREP1) U2OS cells (a) or HeLa cells (b) assessed by qPCR. Error bars show mean ± SEM of three or more independent experiments. c PREP1 binding profile (Big Wig files) to the loci of human SUN1 (upper panel), SUN2 (middle panel) and TMPO/LAP2 (bottom panel) genes in the IGV genome browser are shown. The top bar shows the entire human chromosome 7 (upper panel), human chromosome 22 (middle panel) or human chromosome 12 (bottom panel) with the various chromosomal positions (p and q). The lower corresponding rows show the binding profiles of PREP1 on SUN1 (upper panel), SUN2 (middle panel), or TMPO (LAP2, bottom panel) in Chip-seq experiments using cardiac mesenchymal stromal cells from two human donors and HeLa cells.

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