Fig. 7: Deletion of ORF6 influences the pathogenicity in SARS-CoV-2 in vivo.
From: SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication
a Detection of ORF6 in recombinant SARS-CoV-2 control (Nluc-2A-ORF6) or ΔORF6. VeroE6/TMPRSS2 cells were infected with SARS-CoV-2 WT (Nluc-2A-ORF6) or ΔORF6 and the cell lysates were collected at 24 h post infection. Cell lysates were subjected to western blotting, and then detected the proteins using specific antibodies for ORF6, NP, or Actin. NIID: 2019-nCoV/Japan/TY/WK-521/2020 strain was isolated at the National Institute of Infectious Diseases. Values are kDa. b Percent body weight changes were calculated for all hamsters infected with SARS-CoV-2 WT (blue circle) or ΔORF6 (red triangle). Data are mean ± SD from four independent animals. c Viral RNA in lung homogenates from hamsters was quantified using qRT-PCR (n = 4). *P < 0.05, two-tailed Student’s t-test. error bars represent SD. d Immunohistochemistry of SARS-CoV-2 antigen (NP protein) in lung lobes of hamster infected with SARS-CoV-2 WT or ΔORF6, respectively. Scale bars: 100 μm.
