Fig. 2: opn-SATORI. | Communications Biology

Fig. 2: opn-SATORI.

From: Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis

Fig. 2

a Schematic illustration of opn-SATORI. Biotin-labeled LtrCas13a-crRNA-tgRNA complexes were attached to streptavidin-coated magnetic beads and enriched in microchambers by a magnetic force before the sealing with oil. b Representative images obtained with or without magnetic bead treatment at different concentrations of the SARS-CoV-2 N-gene. c The number of positive chambers obtained using different concentrations of SARS-CoV-2 N-gene or whole genome RNA. The solid lines indicate linear regressions. The values of the background mean + 3 S.D. are indicated by dotted lines. d Time courses of LoD values with or without the magnetic bead treatment, for detection of the SARS-CoV-2 N-gene or whole genome RNA. The dotted line indicates the Ct value of 37 for the SARS-CoV-2 whole genome RNA evaluated by RT-qPCR. (n = 3 technical replicates for c and d).

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