Fig. 3: Discrimination of the S-gene mutations of SARS-CoV-2 variants. | Communications Biology

Fig. 3: Discrimination of the S-gene mutations of SARS-CoV-2 variants.

From: Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis

Fig. 3

a–d Design and screening of crRNA for detection of the N501Y (a), E484K (b), L452R (c), and Q493R/G496S/Q498R (d) mutations in the SARS-CoV-2 S-gene (n = 3 technical replicates). e Discrimination of S-gene mutations in the SARS-CoV-2 variants using opn-SATORI (n = 5 technical replicates). Wuhan-Hu1, B.1.1.7, B.1.351, B.1.617, and B.1.1.529 were used as WT, α, β, δ, and ο variants, respectively. The definition of having a mutation is that the ratio value is greater than 1.0. In the box plots, the boundary of the box closest to zero indicates the 25th percentile, and black line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. The error bars indicate S.D. f Detection specificity of opn-SATORI. All data were obtained at 3 min after oil sealing.

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