Table 1 Properties of higher scFv secreting strains obtained from the genome-wide screen.

From: A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii

Clone name

Disrupted gene

Locus tag

Protein ID

Gene product

Strain name**

50D5

XRN1

CHR3-0780

CCA39734

5’−3’ Exoribonuclease 1

RD-1

188F8

BST1

CHR2-0580

CCA38267

Glycosyl phosphatidyl inositol deacylase

RD-2

164H11

RKR1

CHR1-1469

CCA37582

E3 ubiquitin-protein ligase

RD-3

53A6, 141C11

HIR2

CHR2-0260

CCA37956

HIR (histone regulatory) complex subunit

RD-4

40D3

BUD2

CHR3-0544

CCA39503

GTPase activating factor

RD-5

146F6

SWF1

CHR2-1252

*

Palmitoyltransferase

RD-6

142H2, 183B5

ALD6-1

CHR4-0972

CCA41122

Magnesium-activated aldehyde dehydrogenase, cytosolic

RD-7

121E10

HIR3

CHR2-0471

CCA38159

HIR complex subunit

RD-8

71E11, 83G9, 167H7

DYN1

CHR2-0532

*

Dynein heavy chain

RD-9

22H9

NUM1

CHR1-0804

CCA36944

Mitochondria-ER cortex anchor component

–

86G2, 97C4

HypoP-1

CHR1-0785

CCA36925

Hypothetical protein

–

97G7

HypoP-2

CHR1-1238

CCA37362

Hypothetical protein

–

71B8

SPT6

CHR4-0688

CCA40846

Nucleosome remodeling protein

–

84G11

Not determined

    

21A12

Not determined

    
  1. *Not identified at NCBI (https://www.ncbi.nlm.nih.gov/).
  2. ** Re-constructed Komagataella phaffii dnl4his4 strains carrying scFv expression cassettes with gene deletions. Nine deletion strains were obtained in this study.
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