Fig. 1: D-FFOCT imaging and scratch techniques, results of closing (<25 μm) scratch assays.

a Drawing of our custom-built Full-Field OCT system. BS beam-splitter, HPF high-pass filter, PZT piezo-electric translation, TS translation stage (for the sample). b Schematic of the RPE cell layer before and after the scratch assay (from left to right). c Recombined three channels of HSV computation and the three different channels in lower left. The colorbar represents the frequency variations of the sample (Hue channel). d–g Results of the analysis of a closing scratch assay on a primary porcine RPE cell culture (ppRPE). d Beginning and end (i.e., closing) of the imaging of the scratch assay. The dotted white square corresponds to the area used for calculations. e, f Plots of the evolution of scratch width and wound closure over the acquisition, calculated with SAVE Profiler. g Optical flow calculations showing velocity and motion direction. h–k Results of the analysis of a closing scratch assay on a hiRPE cell culture. h Beginning and end (i.e., closing) of the imaging of the scratch assay. i, j Plots of the evolution of scratch width and wound closure over the acquisition, calculated with SAVE Profiler. k Optical flow calculations. (Statistics: see Supplementary Table 1) (scale-bar: 50 μm).