Fig. 3: Immunohistochemistry validation of organelles. | Communications Biology

Fig. 3: Immunohistochemistry validation of organelles.

From: Dynamic full-field optical coherence tomography allows live imaging of retinal pigment epithelium stress model

Fig. 3

Nuclei were labelled with DAPI. Red dashed lines show the scratch direction. a Actin filaments (phalloidine in IH) validation on ppRPE and hiRPE. The left column shows the cell cortex (static) appearing dark in D-FFOCT. The centre column presents microvilli. Their visualisation is easier in D-FFOCT: they appear as red cillias on top of RPE cells. The right column shows filipods on the side of the cells close to the wound, appearing in red in D-FFOCT such as microvilli. (The IH images for filipods were taken at slightly different depths, showing more stress fibres for ppRPE). b Comparison of pigment granules on both ppRPE (highly pigmented) and hiRPE (few pigments). Actin filaments (cytoskeleton) are labelled with phalloidine in IH, while pigments were imaged with phase contrast imaging (Normarski). c Golgi apparatus (TGN46 in cyan), lysosomes (CTSD in magenta) and mitochondria (coxIV in yellow) IH comparison between normal cell and stressed cell (circled in yellow). d Mitochondria (ATPs) and lysosome (LAMP1) validation in IH between normal cell and stressed cell (circled in yellow). e Related D-FFOCT image for (c) and (d) showing both normal cells and stressed cells (circled in yellow). IH: immunohistochemistry (scale-bar: 10 μm).

Back to article page