Fig. 2: PRM and ELISA verification of the top candidates for differentially expressed proteins. | Communications Biology

Fig. 2: PRM and ELISA verification of the top candidates for differentially expressed proteins.

From: Macrophage-derived exosomal aminopeptidase N aggravates sepsis-induced acute lung injury by regulating necroptosis of lung epithelial cell

Fig. 2

a Heatmap display showing 14 differentially expressed proteins from each group of the independent samples. b Using PRM, a box plot depicts the log2 sum fragment area of discovered peptides from seven targeted proteins. *P < 0.05, ** P < 0.01. c The Log2 summed intensity values for peptides identified with PRM for each specified protein were used in ROC analysis to compare the patients to the controls. d Compared with individuals in healthy control and sepsis patients, plasma exosomal APN and CRP were expressively enhanced in patients with sepsis-induced ALI (P < 0.0001). e According to the ROC curve of plasma exosome biomarkers, APN has a significant predictive ability of sepsis-induced ALI: AUCAPN > AUCCRP (0.839 vs. 0.781). ROC, receiver operating characteristic. AUC, area under the curve.

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