Fig. 1: Distribution of ARGs and MGEs in persistence GWI mouse models.
From: Host gut resistome in Gulf War chronic multisymptom illness correlates with persistent inflammation
a Box plots showing sum relative abundance (%) of selected antibiotic resistance genes (ARGs) in Control (adult C57BL/6 J mice administered with vehicle, n = 11 biologically independent samples), GWI (adult C57BL/6 J mice administered with GW chemicals, n = 11 biologically independent samples) and GWI_FMT (adult C57BL/6 J mice group exposed to GW chemicals followed by Fecal Microbiota Transfer (FMT) treatment, n = 6 biologically independent samples). Median values of 4.38% in Control, 3.12% in GWI, and 4.37% in GWI_FMT indicated by solid black lines. b Procrustes rotation analysis comparing resistome (blue) and microbiome (red) changes using PCA ordinations, (X-axis: PCA1, Y-axis: PCA2, PROTEST, M2 = 0.4302, p = 0.0001). c PCoA of Bray-Curtis dissimilarity between ARG and Taxa profiles across sample groups. Principal coordinate axes PCoA1 (34%) and PCoA2 (15%) are calculated to explain 49% of variance detected. Lines connect points from the center of gravity of each sample group. d Box plots showing sum relative abundance (%) of selected mobile genetic elements (MGEs) Control, GWI and GWI_FMT. Boxes in the box plots indicate interquartile range. Error bars in the box plots extend to the most extreme values within 1.5 times the interquartile range. Outlier data points are represented by black dots drawn outside of the box plot. p-values were determined using PROTEST or Welch’s t-test where p < 0.05 was considered statistically significant.
