Fig. 2: SARS-CoV-2 infection experiments in BO.
From: Cell response analysis in SARS-CoV-2 infected bronchial organoids
a BO were infected with SARS-CoV-2 (1.3 × 105 TCID50/well) in the presence or absence of 10 μM camostat and then cultured with differentiation medium for 5 days. b Immunohistochemistry analysis of SARS-CoV-2 Spike protein (SP) in the infected BO. b is representative of three independent experiments. c The amount of infectious virus in the supernatant was measured using the TCID50 assay. d Parametric Gene Set Enrichment Analysis (PGSEA) applied to GO biological process gene sets was performed in uninfected BO (mock), infected BO (control), and infected BO treated with 10 μM camostat (camostat). Three technical repeats were performed per sample for the RNA-seq analysis. e The amount of infectious virus in the supernatant of spherical and suspended BO was measured using the TCID50 assay. In panels c and e, statistical significance was evaluated by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc tests (*P < 0.05). In panels c and e, data represent mean ± SD from three independent experiments.
