Fig. 3: SARS-CoV-2 infection experiments in BO-ALI.
From: Cell response analysis in SARS-CoV-2 infected bronchial organoids
a BO-ALI were infected with SARS-CoV-2 (1.3 × 105 TCID50/well) and then cultured with differentiation medium for 2 days. b Immunofluorescence analysis of KRT5 (red) and acetylated α-tubulin (green) in uninfected BO-ALI. Nuclei were counterstained with DAPI (blue). c Immunofluorescence analysis of ACE2 (green) and acetylated α-tubulin (red) in uninfected BO-ALI. Nuclei were counterstained with DAPI (blue). d The amount of infectious virus in the supernatant of infected BO or BO-ALI was measured by the TCID50 assay. Statistical significance was evaluated using one-way ANOVA followed by Tukey’s post hoc test (*P < 0.05). Data represent the mean ± SD from three independent experiments. e Immunofluorescence analysis of SARS-CoV-2 Spike protein (SP) (green) and acetylated α-tubulin (red) in uninfected BO-ALI 2 days after the infection. Immunofluorescence analysis of SARS-CoV-2 SP (green) and KRT5 (red) in uninfected BO-ALI. Nuclei were counterstained with DAPI (blue) 2 days after the infection. f Immunofluorescence analysis of SARS-CoV-2 SP (green) and acetylated α-tubulin (red) in infected BO-ALI 7 days after the infection. Immunofluorescence analysis of SARS-CoV-2 SP (green) and KRT5 (red) in infected BO-ALI 7 days after the infection. Nuclei were counterstained with DAPI (blue). g Immunofluorescence analysis of KRT5 (red) and acetylated α-tubulin (green) in BO-ALI 15 days after the infection. Nuclei were counterstained with DAPI (blue). Panels b, c, e–g are representative of three independent experiments.
