Fig. 1: Nox expression and ROS production in bone cells during their differentiation.
From: Nox4 expression in osteo-progenitors controls bone development in mice during early life
a Representative cell culture pictures of ALP staining of ST2 cells in the osteoblast differentiation medium on day 1, day 3, day 5, and day 7. b ST cell proliferation in the osteoblast differentiation medium on day 1, day 3, day 5, and day 7. c ROS generation from ST2 cells cultured in the osteoblast differentiation medium on day 1, day 3, day 5, and day 7. d Representative cell culture pictures of TRAPase staining of RAW264.7 cells in the presence of 30 ng/ml of RANKL on day 1, day 3, and day 5. e RAW264.7 cell proliferation on day 1, day 3, and day 5 in the presence of 30 ng/ml of RANKL. f ROS generation from RAW264.7 cells cultured in the presence of 30 ng/ml of RANKL on day 1, day 3, and day 5. Real-time PCR determined (g) Nox4, (h) Nox1, (i) Nox2 and (j) ALP mRNA expression in ST2 cells cultured in osteoblast differentiation medium on day 1, day 3, day 5, and day 7, six culture wells of 12-well culture plates per group. *p < 0.05 by one-way ANOVA followed by Tukey’s post hoc test compared with Day 1. For each cell line, the results are from one cell-culture experiment with each treatment given to 6 wells.
