Fig. 3: Nox4 gene deletion has minimal effects on osteoclastogenesis.
From: Nox4 expression in osteo-progenitors controls bone development in mice during early life
a Non-adherent bone marrow cells were cultured in the presence of 30 ng/ml RANKL for 6 days. Pictures are showing TRAPase staining of cells from Nox4fl/fl control, PrxCre+/- Nox4fl/fl CKO and Nox4-/- mice with four wells of 96-well plat for cells isolated from each mouse, numbers on bottom of each well are experimental animal numbers. b A typical area of TRAPase pink stained multi-nuclear osteoclasts from non-adherent bone marrow cells from each of the three genotypes. c TRAPase-positive multi-nuclear osteoclasts were counted from each well (four wells per mouse cells) of cultures from each Nox4 genotype. d–f Real-time PCR shows relative mRNA expression of NFaTc1, NFκB, and β-Catenin in total RNA isolated from cultures of non-adherent bone marrow cells from Nox4fl/fl control, PrxCre+Nox4fl/fl CKO, and Nox4-/- mice, three mice per group. Significant differences indicated by p < 0.05, by one-way ANOVA followed by Tukey’s post hoc test.
