Fig. 4: Cell proliferation and ROS generation of bone marrow stromal cells from Nox4-depleted mice catch up to cells from control mice after 10 passages.
From: Nox4 expression in osteo-progenitors controls bone development in mice during early life
a Representative pictures of hematoxylin blue stained cell culture wells of triplicates of passage 3 cells from Nox4fl/fl control, PrxCre+/- Nox4fl/fl CKO, and Nox4-/- mice. b Cell proliferation of cells from culture wells of triplicates of passage 3 cells from mice of the three genotypes. c ROS generation from cells of cultured wells of triplicates of passage 3 cells from mice of the three genotypes. d Representative pictures of hematoxylin blue stained cell culture wells of triplicates of passage 10 cells of the three Nox4 genotypes. e Cell proliferation of cells from culture wells of triplicates of passage 10 cells. f ROS generation from cells of cultured wells of triplicates of passage 10 cells. Significant differences indicated by ***p < 0.001; ****p < 0.0001, by one-way ANOVA followed by Tukey’s post hoc test.
