Fig. 6: Micro-CT bone phenotype of femurs of 32-week-old Nox4fl/fl control, and PrxCre+/-Nox4fl/fl CKO and Nox4-/- mice.
From: Nox4 expression in osteo-progenitors controls bone development in mice during early life
a, b Representative micro-CT images of sagittal and transverse views of the distal femur from one sample from each group of male mice; upper panel shows sagittal view and lower panel shows transverse view. White lines and dots indicates trabecular or cortical bone tissues. c, d H&E and TRAPase histological stainings of the distal femur from one sample from each group of male mice. e–j Micro-CT measures of six parameters from trabecular femoral bone from 32-week-old male mice. BV/TV, bone volume/total tissue volume; BV, bone volume; BS, bone surface; BS/TV, bone surface density; Tb.N, trabecular number; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation; BMD, connective tissue bone mineral density. k, l Representative micro-CT images of the distal femur from one sample from each group of female mice. m, n H&E and TRAPase histological stainings of the distal femur from one sample from each group of female mice. o–t Micro-CT measures of six parameters from trabecular femoral bone from 32-week-old female mice. Parameter BV/TV, BS/TV, Tb.N, Tb.Th, Tb.Sp, and BMD were presented. Data are expressed as mean ± SD, analyzed by one-way ANOVA, additionally, *p < 0.05, **p < 0.01, ***p < 0.001, by Tukey’s multiple comparison. There were 6–9 mice per group.
