Fig. 3: ML protected HUVECs from TBHP induced injury in a mitophagy-dependent manner. | Communications Biology

Fig. 3: ML protected HUVECs from TBHP induced injury in a mitophagy-dependent manner.

From: Activating Parkin-dependent mitophagy alleviates oxidative stress, apoptosis, and promotes random-pattern skin flaps survival

Fig. 3

a, b The protein expression level of LC3-II, p62, Pink1, and Parkin in HUVECs was quantified and showed by a histogram. c, d Expression and quantification of apoptosis-related and oxidative-related proteins Bax, C-caspase3, Bcl-2, HO-1, and SOD1 were measured by western blot. e, f JC-1 probe was performed to detect the change of MMP and was quantified and showed by a histogram (bar: 20 μm). g ATP production in HUVECs was measured by an ATP detection kit and showed by a histogram. All experiments were performed at least 3 times (n ≥ 3). Data are presented as mean ± S.D. n = 3, **P < 0.01, *P < 0.05.

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