Fig. 4: ML promoted mitophagy by upregulating Parkin. | Communications Biology

Fig. 4: ML promoted mitophagy by upregulating Parkin.

From: Activating Parkin-dependent mitophagy alleviates oxidative stress, apoptosis, and promotes random-pattern skin flaps survival

Fig. 4

a M-versus-A (MA) plot was shown the differential gene expression between TBHP and TBHP + ML groups. b Heatmap analyses of the key molecules differentially regulated in HUVECs in control, TBHP, and TBHP + ML groups based on transcriptome analysis. c, d The expression of Parkin was evaluated by western blots in HUVECs treated with si-RNA. e, f Western blot measured the expression level of LC3 and P62 after Parkin knockdown. g, h Expression and quantification of protein Bax, C-caspase3, Bcl-2, HO-1, and SOD1 were measured by western blot. i MitoSox staining was performed to detect the mitochondria ROS in HUVECs (bar: 20 μm). j Detection of ultrastructure of mitochondria and autophagic change by TEM (×10,000 or 50,000) (Black arrow: swollen mitochondria with fractured cristae; Red arrow: autophagolysosome; M: mitochondria; A: autophagosome). k The fluorescence intensity was quantified and demonstrated by a histogram. l ATP production in HUVECs was measured by an ATP detection kit and showed by a histogram. All experiments have been performed at least 3 times (n ≥ 3). Data are presented as mean ± S.D. n = 3, **P < 0.01, *P < 0.05.

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