Fig. 3: Classic Hodgkin lymphoma stained using a combination of tyramide-barcodes and barcode-conjugated primary antibodies.
From: Tyramide-conjugated DNA barcodes enable signal amplification for multiparametric CODEX imaging
a CD30 (1:50 dilution, mouse host, clone Ber-H2, Invitrogen) and MUM1 (1:100 dilution, mouse host, clone MUM1p, Agilent Dako Products) staining was performed using the tyramide-barcodes, which stains the cell membranes and nuclei of Hodgkin cells, respectively (scattered T cells also express MUM1). Other antigens were stained using commercially available barcode-conjugated primary antibodies (Akoya Biosciences, 1:200 dilutions except for CD3e, which was 1:100 dilution). DAPI = blue, CD30 = magenta, MUM1 = yellow, CD68 = red, CD3e = cyan. Staining for CD4, CD8, CD11c, CD20, CD45RO, CD107a, and Ki67 is shown in Fig. 4. b Virtual H&E staining was generated using DAPI and eosin fluorescence images collected after the regular CODEX imaging24. Circles help to identify Hodgkin/Reed-Sternberg cells seen in the fluorescence image and corresponding virtual H&E image.
