Fig. 3: Increased PE-contractility and VGCC alterations. | Communications Biology

Fig. 3: Increased PE-contractility and VGCC alterations.

From: Progressive aortic stiffness in aging C57Bl/6 mice displays altered contractile behaviour and extracellular matrix changes

Fig. 3

Maximal PE contraction in the absence (circles) and presence (squares) of L-NAME (a). Basal NO levels, quantified as the relative increase in PE contraction after addition of L-NAME (b). Contraction-dependent aortic stiffening by addition of 2 µM PE + 300 µm L-NAME at isobaric 80–120 mmHg pressure (c). Maximal PE contractions, as obtained from non-linear regression of the PE concentration-response curves, in the absence (circles) and presence (squares) of 30 nM BAY-K8644 (d), and calculated absolute BAY-K8644 effect (circles, e). Contraction-inhibition by diltiazem in the absence (circles) or presence (squares) of BAY-8644 (f). Statistical analysis using two-way ANOVA (a, d, f), one-way ANOVA (b, c) or one-sample t-test vs. 0 (e). Data are listed as mean ± SEM (a, d–f, n > 8) or each symbol represents an individual biological repeat (b, c, n > 8). Overall significance (bottom of graph) and post hoc significance vs. 4-month value (in graph) are listed. *p < 0.05, **p < 0.01, ***p < 0.001. In d, post hoc comparison for BAY-K8644 effect is listed as #p < 0.05, ##p < 0.01.

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