Fig. 1: Flagellar gene cluster deletion using single or double sgRNAs. | Communications Biology

Fig. 1: Flagellar gene cluster deletion using single or double sgRNAs.

From: Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation

Fig. 1

a, b Design and comparison between single and double sgRNAs. The right-angle arrows and “T” symbols represent promoters and terminators, respectively. c Designed positions of homologous arms and sgRNAs for flagellar gene cluster deletion (left). Editing efficiency of deleting various flagellar gene cluster with single or double sgRNAs (right). 32 colonies in each group were verified by PCR. Homozygous genotype (purple) means the deletion of flagellar cluster in mutants which produce one PCR product with the size of 2.4 kb. Heterozygous genotype (yellow) represents the occurrence of both wild type and deletion of flagellar cluster in mutants which produce two PCR products with the size of 2.4 and 1.7 kb, respectively. The electrophoresis results of PCR products were shown in Supplementary Fig. 1. H1, the upstream homologous arm of flagellar gene cluster; H20 (H30, H40, and H50), the downstream homologous arm of flagellar gene cluster for the deletion of 20 kb (30, 40, and 50 kb) fragment.

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