Fig. 6: Deficiency of O-antigen is the key factor of self-flocculation. | Communications Biology

Fig. 6: Deficiency of O-antigen is the key factor of self-flocculation.

From: Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation

Fig. 6

a O-antigen biosynthesis genes in the PS4 cluster. b Self-flocculation of TD1.0, ΔPS124, ΔPS124/pSEVA321-Part3 and ΔO-antigen after standing for 30 min and their mucoid/dry phenotype. The strains were cultured in LB60 medium for 24 h and used for self-flocculation test (left). Morphology of TD1.0, ΔPS124, ΔPS124/pSEVA321-Part3, and ΔO-antigen cultured on LB60 agar plate (right). TD1.0, H. bluephagenesis TD1.0; ΔPS124, H. bluephagenesis TD1.0 with deletion of PS1, PS2, and PS4 gene clusters; ΔPS124/pSEVA321-Part3, ΔPS124 complemented with Part3 of the PS4 cluster; ΔO-antigen, TD1.0 with deletion of the 7 genes in Part3. c Diagram of the structure of lipopolysaccharide (LPS) in E. coli28. The action positions of WaaL and Wzz are depicted with arrows. GlcN, N-acetylglucosamine; Kdo, 3-deoxy-D-manno-octulosonate; Hep, heptose; Glc, glucose; Gal, galactose; Oligosaccharide, oligosaccharide unit of O-antigen; P, phosphate group.

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