Fig. 9: Illustration of the deletion of EPSs and O-antigen made H. bluephagenesis self-flocculating and electrotransformation feasible. | Communications Biology

Fig. 9: Illustration of the deletion of EPSs and O-antigen made H. bluephagenesis self-flocculating and electrotransformation feasible.

From: Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation

Fig. 9

a EPSs and O-antigen synthesis gene clusters of H. bluephagenesis TD1.0 were deleted with dual-sgRNA CRISPR/Cas strategy, and resulted in H. bluephagenesis ΔPS124. b H. bluephagenesis ΔPS124 can self-flocculate in the range of 0.4–1.0 M NaCl concentration, while H. bluephagenesis ΔPS124 cannot. c H. bluephagenesis ΔPS124 can be transformed by electroporation, whereas H. bluephagenesis ΔPS124 cannot.

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