Table 1 Electrotransformation efficiency of TD1.0 and ΔPS1234Δ50k under different electroporation media and voltages.

From: Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation

Strain

Voltage (kV)

Transformation efficiency under different electroporation medium (cfu/μg DNA)

1.0 M sorbitol 0.5 M trehalose

0.5 M sorbitol 0.5 M mannose 0.5 M trehalose

1.0 M sucrose

0.5 M sucrose

TD1.0

2.5

0

0

0

0

1.8

-

-

0

0

ΔPS1234Δ50k

2.5

2.7 ± 4.6

1.3 ± 1.2

2.0 ± 2.0

320.7 ± 71.3

1.8

-

-

0

20.7 ± 1.2

  1. -: The voltage was not considered. Three parallels were set for each group.
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