Fig. 4: Cell type-specific expression of GBA variants.
From: Redefining GBA gene structure unveils the ability of Cap-independent, IRES-dependent gene regulation
a Comparison of the expression levels of GBA transcripts in fibroblasts. The expression levels of GBA transcripts were normalized to those of GAPDH, and expressed as the relative quantity to that of DF2. Each experiment was performed in triplicate. DF, dermal fibroblasts; OF, oral mucosa fibroblasts. b Expression levels of GBA transcripts among different cell types using an in vitro hematopoietic differentiation system of HL60 cells. The expression levels of GBA were normalized to those of PPIA, and expressed as the relative quantity to that of control HL60 cells. Each experiment was performed in triplicate except v6/7, N in v3, C in total GBA were duplicate. C, control HL60 cells (no treatment); M, iMacs; N, iNeuts. c The expression levels of GCase protein by western blot analysis. Representative western blot analysis data is shown. C1 and C2, two individual controls (nontreated HL60 cells); M, iMacs; N, iNeuts, P, positive control (human GBA in pCIneo-transfected HEK293 cell lysate). d GCase activity. C, control HL60 cells (no treatment); M, iMacs; N, iNeuts. Each experiment was performed in triplicate except control (control, n = 6). **p < 0.01. Data represent the mean ± STDEV.
