Fig. 6: IRES activity in the 5’ UTRs of GBA variants.
From: Redefining GBA gene structure unveils the ability of Cap-independent, IRES-dependent gene regulation
a Formula for estimation of IRES activity by bicistronic reporter assay and the reporter construct. The 5’ UTRs of GBA variants (pink box) were inserted into the pEF1a-RF vector. EF1a, EF1α promoter; hRL, Renilla luciferase CDS; FL, firefly luciferase CDS. b IRES activity in OFs. c IRES activity in HL60-derived macrophages (HL60 + PMA). Gray bar, 20 nM PMA; black bar, 40 nM PMA. d IRES activity in OFs treated with 3 nM PMA. e Effects of PMA on IRES activity in OFs. White bar, no treatment; gray bar, 3 nM PMA; black bar, 10 nM PMA. Each experiment was performed in triplicate. *p < 0.05. Data represent the mean ± STDEV.
