Fig. 7: GCase translation in OF2 cells after rapamycin treatment.
From: Redefining GBA gene structure unveils the ability of Cap-independent, IRES-dependent gene regulation
a Diagram of sample preparation. OF2 cells were precultured in media with 0.5% FBS for 24 h before rapamycin treatment. The cells were treated rapamycin for 1 h, FBS was added up to 10% and maintained for 48 h. b Results of GCase expression in rapamycin-treated OF2 cells, as determined by western blot analysis. EtOH; 0.1% ethanol, FBS stim.; FBS stimulation. c Confirmation of the GCase expression remove its glycosylation. Each experiment was performed in the triplicate and representative data was shown.
