Fig. 8: Analyses of the GBA 3’ end.
From: Redefining GBA gene structure unveils the ability of Cap-independent, IRES-dependent gene regulation
a Summary of GBA 3’-RACE. The top 2 lines indicate the known structures of J03059 and GBA variants v1 to v5. #1–3 are the results of this study. The boxes indicate the exons. Gray, coding region; white, UTR. The number is the size of exon 13. In the middle, the positions of putative poly (A) addition signals are indicated in blue (Horowitz, et al.8) and red (Beaudoing et al.32). b In silico analysis of miRNA binding to GBA. Red, miR22-3p based on Straniero et al.10; black, database search results obtained using miRDB. c, d Gene expression of GBA, GBAP1 and miR22-3p in fibroblasts (c) and HL60 derivatives (d). The qPCR results for GBA and GBAP1 were normalized to 18 S rRNA expression, whereas miR22-3p was normalized to RNU6 expression, respectively. In d, after normalized expression value, calibrated as a relative quantity to no-treated HL60 (C1). Black bar, GBA; white bar, GBAP1; gray bar, miR22-3p. Each experiment was performed in triplicate. Data represent the mean ± STDEV.
