Fig. 1: Distributions of miscounting between foci detected and the number of DSBs within the central microscope slice of the cell nucleus when using a Ku/DNA-PKcs marker of fluorescence.

Each panel is the same four radiation types and six-time points being compared for different radiation doses, where a–d correspond to 1.0, 2.0, 5.0, 10.0 Gy respectively. Error bars correspond to 1.5 times the interquartile range in either direction. Mann–Whitney test between different radiation types at each time point and dose to highlight statistically significant differences. p values have been adjusted using the Bonferroni correction. Significance notation refers to the following thresholds: ns = p > 0.05, *p > 0.01, **p > 1e−3, ***p > 1e−4, ****p < 1e−4. There are 200 samples for each box-plot. All microscope images have been emulated using the Airyscan ×63 point spread function.